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小鼠胰島素樣生長(zhǎng)因子1酶免試劑盒,(IGF-1)ELISA檢測(cè)試劑盒,英文名:IGF-1 ELISA Kit,英文縮寫:IGF-1 ,常見(jiàn)試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠胰島素樣生長(zhǎng)因子1ELISA檢測(cè)試劑盒,IGF-1 檢測(cè)試劑盒,IGF-1 檢測(cè)試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠胰島素樣生長(zhǎng)因子1酶免試劑盒,(IGF-1)ELISA檢測(cè)試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對(duì)標(biāo)本進(jìn)行預(yù)處理,例如對(duì)血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測(cè)定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測(cè)中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對(duì)競(jìng)爭(zhēng)法及定量檢測(cè)影響很大,不注意可能會(huì)導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過(guò)程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無(wú)關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過(guò)程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測(cè)讀儀(酶標(biāo)儀),比色法判讀可選擇單波長(zhǎng)或雙波長(zhǎng),根據(jù)反應(yīng)液顏色的不同選用不同波長(zhǎng)的濾光片,以空白孔校零測(cè)定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。CD83分子(CD83)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cluster Of Differentiation 83 (CD83)48T/96TY10695Rattus norvegicus (Rat)
睫狀神經(jīng)營(yíng)養(yǎng)因子(CNTF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Ciliary Neurotrophic Factor (CNTF)48T/96TY10696Mus musculus (Mouse)
血管內(nèi)皮細(xì)胞蛋白C受體(PROCR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Protein C Receptor, Endothelial (PROCR)48T/96TY10697Rattus norvegicus (Rat)
內(nèi)分泌腺來(lái)源血管內(nèi)皮生長(zhǎng)因子(EG-VEGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)48T/96TY10698Rattus norvegicus (Rat)
內(nèi)分泌腺來(lái)源血管內(nèi)皮生長(zhǎng)因子(EG-VEGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Endocrine Gland Derived Vascular Endothelial Growth Factor (EG-VEGF)48T/96TY10699Mus musculus (Mouse)
嗜酸粒細(xì)胞趨化因子(ECF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Eosinophil Chemotactic Factor (ECF)48T/96TY10700Rattus norvegicus (Rat)
紅細(xì)胞生成素受體(EPOR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Erythropoietin Receptor (EPOR)48T/96TY10701Rattus norvegicus (Rat)
紅細(xì)胞生成素受體(EPOR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Erythropoietin Receptor (EPOR)48T/96TY10702Mus musculus (Mouse)
E選擇素(SELE)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Selectin, Endothelium (SELE)48T/96TY10703Oryctolagus cuniculus (Rabbit)
紅細(xì)胞生成素(EPO)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Erythropoietin (EPO)48T/96TY10704Rattus norvegicus (Rat)
凋亡相關(guān)因子(FAS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Factor Related Apoptosis (FAS)48T/96TY10705Rattus norvegicus (Rat)
凋亡相關(guān)因子配體(FASL)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Factor Related Apoptosis Ligand (FASL)48T/96TY10706Rattus norvegicus (Rat)
酸性成纖維細(xì)胞生長(zhǎng)因子(FGF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)48T/96TY10707Rattus norvegicus (Rat)
酸性成纖維細(xì)胞生長(zhǎng)因子(FGF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 1, Acidic (FGF1)48T/96TY10708Mus musculus (Mouse)
干擾素α(IFNα)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY10709Sus scrofa; Porcine (Pig)
干擾素α(IFNα)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interferon Alpha (IFNa)48T/96TY10710Oryctolagus cuniculus (Rabbit)
成纖維細(xì)胞生長(zhǎng)因子6(FGF6)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 6 (FGF6)48T/96TY10711Rattus norvegicus (Rat)
成纖維細(xì)胞生長(zhǎng)因子4(FGF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY10712Mus musculus (Mouse)
成纖維細(xì)胞生長(zhǎng)因子4(FGF4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 4 (FGF4)48T/96TY10713Rattus norvegicus (Rat)
成纖維細(xì)胞生長(zhǎng)因子6(FGF6)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 6 (FGF6)48T/96TY10714Mus musculus (Mouse)
成纖維細(xì)胞生長(zhǎng)因子9(FGF9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY10715Rattus norvegicus (Rat)
成纖維細(xì)胞生長(zhǎng)因子9(FGF9)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Fibroblast Growth Factor 9 (FGF9)48T/96TY10716Mus musculus (Mouse)
FMS樣酪氨酸激酶3(Flt3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for FMS Like Tyrosine Kinase 3 (Flt3)48T/96TY10717Rattus norvegicus (Rat)
中性粒細(xì)胞激活蛋白3(NAP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neutrophil Activating Protein 3 (NAP3)48T/96TY10718Rattus norvegicus (Rat)
中性粒細(xì)胞激活蛋白3(NAP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Neutrophil Activating Protein 3 (NAP3)48T/96TY10719Mus musculus (Mouse)
粒細(xì)胞集落刺激因子(GCSF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Colony Stimulating Factor 3, Granulocyte (GCSF)48T/96TY10720Rattus norvegicus (Rat)
膠質(zhì)細(xì)胞系來(lái)源神經(jīng)營(yíng)養(yǎng)因子(GDNF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glial Cell Line Derived Neurotrophic Factor (GDNF)48T/96TY10721Mus musculus (Mouse)
生長(zhǎng)激素(GH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Hormone (GH)48T/96TY10722Rattus norvegicus (Rat)
生長(zhǎng)激素(GH)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Growth Hormone (GH)48T/96TY10723Mus musculus (Mouse)
糖蛋白130(gp130)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Glucoprotein 130 (gp130)48T/96TY10724Rattus norvegicus (Rat)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY10725Rattus norvegicus (Rat)
肝細(xì)胞生長(zhǎng)因子(HGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Hepatocyte Growth Factor (HGF)48T/96TY10726Mus musculus (Mouse)
胰島素樣生長(zhǎng)因子結(jié)合蛋白1(IGFBP1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 1 (IGFBP1)48T/96TY10727Rattus norvegicus (Rat)
胰島素樣生長(zhǎng)因子結(jié)合蛋白2(IGFBP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 2 (IGFBP2)48T/96TY10728Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子D(VEGFD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD)48T/96TY10729Rattus norvegicus (Rat)
胰島素樣生長(zhǎng)因子結(jié)合蛋白3(IGFBP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 3 (IGFBP3)48T/96TY10730Rattus norvegicus (Rat)
胰島素樣生長(zhǎng)因子結(jié)合蛋白4(IGFBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)48T/96TY10731Mus musculus (Mouse)
胰島素樣生長(zhǎng)因子結(jié)合蛋白4(IGFBP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Insulin Like Growth Factor Binding Protein 4 (IGFBP4)48T/96TY10732Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子D(VEGFD)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor D (VEGFD)48T/96TY10733Mus musculus (Mouse)
白介素11(IL11)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 11 (IL11)48T/96TY10734Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子C(VEGFC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor C (VEGFC)48T/96TY10735Rattus norvegicus (Rat)
白介素12B(IL12B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY10736Mus musculus (Mouse)
血管內(nèi)皮生長(zhǎng)因子C(VEGFC)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor C (VEGFC)48T/96TY10737Mus musculus (Mouse)
白介素12B(IL12B)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 12B (IL12B)48T/96TY10738Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子B(VEGFB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB)48T/96TY10739Rattus norvegicus (Rat)
白介素12A(IL12A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Interleukin 12A (IL12A)48T/96TY10740Rattus norvegicus (Rat)
血管內(nèi)皮生長(zhǎng)因子B(VEGFB)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Vascular Endothelial Growth Factor B (VEGFB)48T/96TY10741Mus musculus (Mouse)
尿激酶型纖溶酶原激活因子受體(uPAR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)48T/96TY10742Rattus norvegicus (Rat)
尿激酶型纖溶酶原激活因子受體(uPAR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator, Urokinase Receptor (uPAR)48T/96TY10743Mus musculus (Mouse)
尿激酶型纖溶酶原激活因子(uPA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator, Urokinase (uPA)48T/96TY10744Rattus norvegicus (Rat)
尿激酶型纖溶酶原激活因子(uPA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Plasminogen Activator, Urokinase (uPA)48T/96TY10745Mus musculus (Mouse)
血小板生成素(TPO)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Thrombopoietin (TPO)48T/96TY10746Rattus norvegicus (Rat)
腫瘤壞死因子β(TNFβ)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tumor Necrosis Factor Beta (TNFb)48T/96TY10747Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子4(TIMP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY10748Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子4(TIMP4)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)48T/96TY10749Mus musculus (Mouse)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY10750Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子3(TIMP3)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)48T/96TY10751Mus musculus (Mouse)
組織金屬蛋白酶抑制因子2(TIMP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY10752Rattus norvegicus (Rat)
組織金屬蛋白酶抑制因子2(TIMP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tissue Inhibitors Of Metalloproteinase 2 (TIMP2)48T/96TY10753Mus musculus (Mouse)
內(nèi)皮細(xì)胞TEK酪氨酸激酶(Tie2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for TEK Tyrosine Kinase, Endothelial (Tie2)48T/96TY10754Rattus norvegicus (Rat)
免疫球蛋白樣EGF樣域酪氨酸激酶1(Tie1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY10755Rattus norvegicus (Rat)
免疫球蛋白樣EGF樣域酪氨酸激酶1(Tie1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains Protein 1 (Tie1)48T/96TY10756Mus musculus (Mouse)
基質(zhì)細(xì)胞衍生因子1(SDF1)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stromal Cell Derived Factor 1 (SDF1)48T/96TY10757Rattus norvegicus (Rat)
干細(xì)胞因子受體(SCFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stem Cell Factor Receptor (SCFR)48T/96TY10758Rattus norvegicus (Rat)
干細(xì)胞因子受體(SCFR)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Stem Cell Factor Receptor (SCFR)48T/96TY10759Mus musculus (Mouse)
CD40配體(CD40L)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cluster Of Differentiation 40 Ligand (CD40L)48T/96TY10760Rattus norvegicus (Rat)
CD30配體(CD30L)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Cluster Of Differentiation 30 Ligand (CD30L)48T/96TY10761Rattus norvegicus (Rat)
調(diào)節(jié)激活正常T-細(xì)胞表達(dá)分泌因子(RANTES)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) ELISA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)48T/96TY10762Rattus norvegicus (Rat)
胎盤生長(zhǎng)因子(PLGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Placenta Growth Factor (PLGF)48T/96TY10763Rattus norvegicus (Rat)
胎盤生長(zhǎng)因子(PLGF)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)ELISA Kit for Placenta Growth Factor (PLGF)48T/96TY10764Mus musculus (Mouse))
各種類型ELISA測(cè)定時(shí), 一般需經(jīng)過(guò)以下步驟:固相包被→加待測(cè)樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。